Aug 01, 2012 |
Crystal clarity for protein molecular structure
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(Nanowerk News) An EU-funded initiative has integrated Europe's software and infrastructure resources to create a high-tech platform for use in the area of structural genomics.
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Biological crystallography (biocrystallography) creates precise three-dimensional (3D) architectural models of biological molecules. As molecular interactions depend on structural sites fitting together, the design of a molecule is key to its function. Armed with a molecule's structure, scientists can predict with accuracy which molecules will interact and what drugs will affect mode of operation.
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To maximise the state of the art in biocrystallography, the 'Biocrystallography (X) on a highly integrated technology platform for European structural genomics' (Bioxhit) project integrated the very best of current technologies at major European centres.
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Bioxhit mobilised all synchrotron facilities with beamlines equipped for macromolecular crystallography. Merged with the cream of software developers in relevant fields, the project aimed to produce a high-throughput pipeline linking the crystallisation of a protein to its 3D structure at an atomic level.
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The progress of 178 project tasks – 141 scientific undertakings and 37 training initiatives – were recorded in detail. Science highlights included coupling crystallisation with X-ray data collection and a high-precision, motorised kappa-goniometer (to measure angles). Sophisticated algorithms were developed and a system of remote data collection was instigated. Comprehensive multi-option structure determination is now available.
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Major emphasis was placed on automation for a consequent reduction in time for production of each structure. Handling samples, detection of X-ray patterns and model building were all adapted for high-precision automation replacing time-consuming manual steps.
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Training activities were a cornerstone of the project. Four advanced training and dissemination centres were created and courses were held at synchrotron centres and communicated to satellite locations to disseminate biocrystallography technologies.
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Bioxhit has contributed to making Europe one of the world leaders in the area of X-ray biocrystallography. Integration of this technology with genomics and proteomics will ensure progress in the area of target drug design.
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